Composite

Part:BBa_K091231:Design

Designed by: Malcolm Campbell   Group: iGEM08_Davidson-Missouri_Western   (2010-08-02)


LuxR producer and XOR gate


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1159
    Illegal AgeI site found at 1271
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2696


Design Notes

This version of the pLac promoter was chosen because the shorter version (55 bp) is unstable and mutates to silence itself.


Source

Ligating existing parts.

References